The effects of high gravitational stress on the expression of stress response genes in Arabidopsis thaliana
DOI:
https://doi.org/10.17307/wsc.v1i1.299Palabras clave:
Arabidopsis, Hypergravity, Botany, Plants, AstrobotanyResumen
As humans venture farther into space, we are increasingly interested in how plants respond to the unique conditions of spaceflight. This experiment focuses on how gene expression in the model plant Arabidopsis thaliana is affected by high-gravitational stress that is comparable to the amount experienced on a small model rocket. The genes chosen have been studied previously for their roles in mechanical stress and pathogen response. Our results showed an increase in expression of the genes TCH2, TCH3, and MPK3, no change in the expression of CBP60g, and a decrease in the expression of RCI3 and ICS1. These results are largely consistent with spaceflight data and warrant further investigation into the effects of rocket launch on plant growth.
Citas
Haya Friedman et al. Inhibition of the Gravitropic Response of Snapdragon Spikes by the Calcium-Channel Blocker Lanthanum Chloride. Plant Physiology Oct 1998, 118 (2) 483-492;
Krishnakumar, V. et al. ThaleMine: a warehouse for Arabidopsis data integration and discovery. Plant and Cell Physiology 2017, 58(1), pp.e4-e4. DOI: 10.1104/pp.118.2.4
Kwon, T., Sparks, J. A., Nakashima, J., Allen, S. N., Tang, Y., & Blancaflor, E. B. (2015). Transcriptional response of Arabidopsis seedlings during spaceflight reveals peroxidase and cell wall remodeling genes associated with root hair development. American Journal of Botany, 102(1), 21-35. doi:10.3732/ajb.1400458
Morpheus: Versatile matrix visualization and analysis software. Broad Institute https://software.broadinstitute.org/morpheus
Nakai, J., Ohkura, M., & Imoto, K. (2001). A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein. Nature Biotechnology, 19(2), 137-141. doi:10.1038/84397
Snel B et al. STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene. Nucleic Acids Res. 2000 Sep 15;28(18)
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
