The search for novel bacteriophages aboard the International Space Station leads to the identification of two Bacillus species
DOI:
https://doi.org/10.17307/wsc.v1i1.223Keywords:
bacteriophages, bacteria, international space station, biocontrol, microbesAbstract
The establishment of a permanent human presence on the International Space Station (ISS) raised new questions regarding the microbial burden inside this closed environment. High-efficiency particulate filters (HEPA) have been installed inside the ISS to reduce the level of harmful microbes that may affect an astronaut’s health. While multiple studies have investigated bacteria within the ISS, no research has been done to detect the viruses that attack bacteria, bacteriophages, which can be used for biocontrol of bacterial levels. Bacteriophages specifically infect bacteria, replicate, and lyse the bacterial host cell, releasing new progeny. The goal of this interdisciplinary project is to isolate and characterize bacteriophages obtained from ISS HEPA filter samples. In the process of isolating bacteriophages, two Bacillus species were recovered and identified from the HEPA filters. This research allows for the potential application of bacteriophages in therapeutics and in the control of microbial burden in space.Â
References
Ackermann, H.W., et al. (1978), Partial characterization of a cubic Bacillus phage. Can J Micobiol., 24, 986–993.
Castro, V. A., et al. (2004), Microbial characterization during the early habitation of the International Space Station. Microb. Ecol., 47, 119–126.
Checinska, A., et al. (2015), Microbiomes of the dust particles collected from the International Space Station and Spacecraft Assembly Facilities. Microbiome, 3, 50.
Clokie, M. R., et al. (2011), Phages in nature. Bacteriophage, 1, 31–45.
Comeau, A. M., et al. (2008), Exploring the prokaryotic virosphere. Res. Microbiol., 159, 306–313.
Danovaro, R., et al. (2011), Marine viruses and global climate change. FEMS Microbiol. Rev., 35, 993–1034.
Coil, DA., et al. (2016), Growth of 48 built environment bacterial isolates on board the International Space Station (ISS). PeerJ, 4:e1842.
D’Herelle, F. (2007), On an invisible microbe antagonistic toward dysenteric bacilli: brief note by Mr. F. D’Herelle, presented by Mr. Roux. 1917. Res. Microbiol. 158, 553–554.
Golec, P., et al. (2011), A reliable method for storage of tailed phages. Journal of Microbiological Methods, 84(3), 486–489.
Kazi, M., and Annapure, U.S., (2016), Bacteriophage biocontrol of foodborne pathogens. Journal of Food Science and Technology., 53(3), 1355-1362.
La Duc, M. T., et al., (2004), Microbial monitoring of spacecraft and associated environments. Microb. Ecol., 47, 150–158.
La Duc, M. T., et al., (2014), A Genetic Inventory of Spacecraft and Associated Surfaces. Astrobiology, 14, 15–23.
Lin, D.M., et al., (2017), Phage therapy: An alternative to antibiotics in the age of multi-drug resistance. World J Gastrointest Pharmacol Ther., 8(3),162.
Luepke, K.H., et al., (2017), Past, Present, and Future of Antibacterial Economics: Increasing Bacterial Resistance, Limited Antibiotic Pipeline, and Societal Implications. Pharmacotherapy, 37(1), 71-84.
McKay, C. P., (1998), Past, present, and future life on Mars. Gravit. Space Biol. Bull., 11, 41–50.
Romig, W.R. and Brodetsky, A.M., (1961), Isolation and preliminary characterization of bacteriophages for Bacillus subtilis. J Bacteriol., 82, 135–141.
Scoon, G. E., et al., (1995), Planetary environment protection ID no: F3.3-M.1.05 implications for the development of a network of surface stations on Mars. Adv. Space Res., 15, 261–272.
Strauch, M.A., et al., (1989), The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J, 8, 1615–1621.
Venkateswaran, K., et al., (2014), International Space Station environmental microbiome - microbial inventories of ISS filter debris. Appl. Microbiol. Biotechnol., 98, 6453–6466.
Venkateswaran, K., et al., (2004), Evaluation of various cleaning methods to remove bacillus spores from spacecraft hardware materials. Astrobiology, 4, 377–390.
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